称取本品36g,加入蒸馏水或去离子水1 L,搅拌加热煮沸至完全溶解,分装三角瓶,121℃灭菌15min。称取0.1g 萘啶酮酸(029992)溶于10ml0.05mol/L氢氧化钠溶液中,另称取0.1g吖啶黄素(029990)溶于10ml灭菌蒸馏水或去离子水中,过滤除菌。使用前225ml培养基中加入萘啶酮酸溶液0.90ml和吖啶黄溶液0.34ml可制成EB增菌液。Suspend 36 g in 1 L of distilled or deionized water.Heat with frequent a