Synonym FCA
MDL number MFCD00131105
General description Each mL contains 1 mg of Mycobacterium tuberculosis
(H37Ra, ATCC 25177), heat killed and dried, 0.85 mL paraffin oil and 0.15 mL
mannide monooleate.
Specificity Freund's Adjuvants may be used to produce water-in-oil emulsions
of immunogens. Antigens in water-in-oil emulsions stimulate high and long-lasting
antibody responses which can be attributed to the slow release of antigen.
Application Antigens (preferably in saline) are typically mixed with an equal volume of
the adjuvant to form an emulsion. See Harlow, E. and Lane, D., Antibodies a Laboratory
Manual, (Cold Spring Harbor Laboratory, 1988) for protocols and information on dosage.
Biochem/physiol Actions The mycobacteria in Complete Freund's adjuvant attracts
macrophages and other cells to the injection site which enhances the immune response.
For this reason, Complete Freund's Adjuvant is used for initial injections and Incomplete
Freund's Adjuvant for subsequent boosts.
Properties
form cell suspension
color amber